General Information
Symbol
Dmel\pygo
Species
D. melanogaster
Name
pygopus
Annotation Symbol
CG11518
Feature Type
FlyBase ID
FBgn0043900
Gene Model Status
Stock Availability
Gene Snapshot
Pygopus is a key nuclear component of the Wnt signaling pathway. It facilitates arm recruitment to Wnt-responsive genes by interacting with the transcription cofactor Chi. It also binds lgs and His3 methylated tail to associate with arm in a process that may function as a transition from gene silence to Wnt-induced transcription. [Date last reviewed: 2016-06-23]
Also Known As
gam, BEST:LD21971
Genomic Location
Cytogenetic map
Sequence location
3R:31,575,835..31,580,699 [-]
Recombination map
3-102
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
-
Summaries
Gene Group Membership
Wnt-TCF Signaling Pathway Core Components -
The canonical Wnt signaling pathway is initiated by the binding of a Wnt ligand to a frizzled family receptor on the cell surface. Activation of the pathway leads to the inhibition of cytoplasmic β-catenin (arm) degradation and its subsequent accumulation in the nucleus, where it regulates the transcription of target genes. (Adapted from FBrf0218499 and FBrf0223299).
WNT ENHANCEOSOME -
The Wnt enhanceosome complex binds to Wnt/wg-enhancer elements via the TCF/LEF protein, pan. In the absence nuclear β-catenin (arm), Wnt/wg-responsive genes are silenced by the repressor gro binding to the enhanceosome. Activation of the canonical Wnt signaling pathway leads to arm accumulation in the nucleus, incorporation into the enhanceosome and the stimulation of transcription. (Adapted from FBrf0235074 and FBrf0229654).
UniProt Contributed Function Data
Involved in signal transduction through the Wnt pathway.
(UniProt, Q9V9W8)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\pygo or the JBrowse view of Dmel\pygo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Gene model reviewed during 5.53
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0085806
4507
815
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085167
80.5
815
8.38
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear ARM/PAN complex.
(UniProt, Q9V9W8)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pygo using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (14 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117
inferred from physical interaction with UniProtKB:P18824,UniProtKB:Q961D9
Terms Based on Predictions or Assertions (0 terms)
Biological Process (10 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117
inferred from genetic interaction with FLYBASE:Axn; FB:FBgn0026597
inferred from genetic interaction with FLYBASE:wg; FB:FBgn0284084
inferred from genetic interaction with FLYBASE:Apc2; FB:FBgn0026598
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from physical interaction with UniProtKB:P18824
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q961D9
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
(assigned by UniProt )
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pygo in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 15 )
Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of pygo
Allele of pygo
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of pygo
Deletions and Duplications ( 6 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
 
 
6 of 15
No
Yes
 
 
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
Yes
5 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
7 of 13
Yes
Yes
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
2 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
9 of 15
Yes
Yes
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No orthologs reported.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190HVY )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150B7Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CZC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CX9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0KKH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
Alleles Reported to Model Human Disease (Disease Ontology)
Download
Models ( 0 )
Allele
Disease
Evidence
References
Interactions ( 1 )
Allele
Disease
Interaction
References
Comments ( 0 )
 
Human Orthologs (via DIOPT v7.1)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear ARM/PAN complex.
(UniProt, Q9V9W8 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
Pathways
Gene Group - Pathway Membership (FlyBase)
Wnt-TCF Signaling Pathway Core Components -
The canonical Wnt signaling pathway is initiated by the binding of a Wnt ligand to a frizzled family receptor on the cell surface. Activation of the pathway leads to the inhibition of cytoplasmic β-catenin (arm) degradation and its subsequent accumulation in the nucleus, where it regulates the transcription of target genes. (Adapted from FBrf0218499 and FBrf0223299).
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-102
Cytogenetic map
Sequence location
3R:31,575,835..31,580,699 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
100C6-100C6
Limits computationally determined from genome sequence between P{PZ}l(3)rM731rM731 and P{EP}pygoEP1076
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
100C6-100C7
100C6-100C7
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (10)
Genomic Clones (19)
cDNA Clones (59)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: pygo gam
    Source for database merge of
    Source for merge of: BEST:LD21971 CG11518
    Source for merge of: gam BEST:LD21971
    Source for merge of: pygo anon-WO02077023.1
    Additional comments
    FlyBase curator comment: "EP3408" overexpression phenotype stated to be due to its effect on pygo but orientation of the P{EP} element suggests otherwise.
    Source for merge of pygo anon-WO02077023.1 was sequence comparison ( date:051113 ).
    Other Comments
    dsRNA made from templates generated with primers directed against this gene.
    dsRNA has been made from templates generated with primers directed against this gene. RNAi of pygo causes a reduction in the arborization of ddaD and ddaE neurons. There is a severe reduction in primary branch outgrowth, lateral branching and lateral branch length. Additionally, there is also inappropriate dendritic routing of ddaE neurons.
    pygo is necessary for apoptosis of excess photoreceptors at the periphery of the developing eye during pupal stages.
    In order to activate pan protein, arm protein must enter the nucleus and form a complex with lgs and pygo proteins.
    Null mutations have been isolated that affect the epidermis, denticle belts, antenna, legs and wings and are recessive viable.
    pygo and lgs are required for wg signal transduction at the level of nuclear arm.
    pygo is required for wg signalling.
    Identification: in a screen to identify P{EP} lines that suppress the small eye phenotype caused by overexpression of wg when coexpressed in the eye.
    pygo inhibits wg signalling when overexpressed and when reduced in activity.
    Identification: transcription unit identified during molecular analysis of the rod genomic region.
    Origin and Etymology
    Discoverer
    Etymology
    A pygopus is a legless lizard with scaly skin.
    Identification
    External Crossreferences and Linkouts ( 43 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Synonyms and Secondary IDs (15)
    Reported As
    Secondary FlyBase IDs
    • FBgn0028951
    • FBgn0039853
    • FBgn0064198
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (122)