General Information
Symbol
Dmel\Met
Species
D. melanogaster
Name
Methoprene-tolerant
Annotation Symbol
CG1705
Feature Type
FlyBase ID
FBgn0002723
Gene Model Status
Stock Availability
Gene Snapshot
Methoprene-tolerant is a bHLH-PAS domain transcription factor paralogous to gce. It is a receptor for the sesquiterpenoid juvenile hormone. [Date last reviewed: 2016-08-18]
Also Known As
Rst(1)JH, DmMet
Genomic Location
Cytogenetic map
Sequence location
X:11,616,124..11,621,280 [+]
Recombination map
1-35
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Summaries
Gene Group Membership
BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -
Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
Rst(1)JH: Resistance (1) Juvenile Hormone
Semidominant mutation conferring 50-100 fold increase in resistance to juvenile hormone III or its analogue methoprene over that of wild type. Also resistant to methoprene-induced tumors and abnormalities in adult cuticle; action of Rst(1)JH autonomous in gynandromorphs. Authors speculate that gene may affect juveline-hormone receptor.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Met or the JBrowse view of Dmel\Met for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Gene model reviewed during 5.53
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0073521
3756
716
FBtr0343585
5088
716
Additional Transcript Data and Comments
Reported size (kB)
5.5, 3.3 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0073368
78.7
716
6.74
FBpp0310182
78.7
716
6.74
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

716 aa isoforms: Met-PA, Met-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
716 (aa); 79 (kD predicted)
Comments
Rst(1)JH protein is specifically competed by juvenile hormone III.
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Met using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (11 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with UniProtKB:O18388
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with FLYBASE:ftz-f1; FB:FBgn0001078
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Expression Data
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Met in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 17 )
Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Met
Allele of Met
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of Met
GAL4 construct
Name
Expression Data
Deletions and Duplications ( 9 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
tarsal segment 1 & joint (with Metatarsi-irregular1)
tarsal segment 2 & joint (with Metatarsi-irregular1)
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
No
3 of 15
Yes
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
No
3 of 15
Yes
No
1 of 15
No
No
 
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
3 of 13
Yes
No
2 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
2 of 12
Yes
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
3 of 15
Yes
No
3 of 15
Yes
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (0)
No orthologs reported.
Arabidopsis thaliana (thale-cress) (0)
No orthologs reported.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904T3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915023J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01VT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0EBI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-35
    Cytogenetic map
    Sequence location
    X:11,616,124..11,621,280 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    10D1-10D1
    Limits computationally determined from genome sequence between P{EP}CG32666EP1452 and P{EP}Ptp10DEP1172
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    10C-10C
    (determined by in situ hybridisation)
    10C5-10C5
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (23)
    Genomic Clones (7)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (17)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: Rst(1)JH CG1705
    Source for database merge of
    Additional comments
    Six EMS-induced, X-linked mutations resistant to juvenile hormone reported by Arking and Vlach (1970) (now lost); resistance factors varied from 1.4 to 21.5 by their methods. Presumed to be alleles of the mutation described by Wilson and Fabian.
    Other Comments
    Ortholog of B. mori juvenile-hormone-related gene (involved in JH biosynthesis, metabolism or signaling).
    Alleles of Rst(1)JH vary in their effects on both uni- and multivariate life history traits.
    Cloning and characterisation of Rst(1)JH.
    Molecular cloning and characterisation of the Met gene region.
    Mutations alter Juvenile hormone reception during late larval development.
    Rst(1)JH gene product performs a role in the regulation of insect larval molting and metamorphosis of the epidermis and the nervous system.
    Distribution of juvenile hormone binding proteins between the cytosol and nucleus was studied by photoaffinity labelling with tritiated epoxyfarnesyl diazoacetate. An 85kD cytosolic juvenile hormone binding protein is suggested to be the same as that identified by Shemshedini et al., (JBC 265:1913--1918 ) alterations in which are correlated with mutations at this locus.
    Located in 10C2-10D4 by deficiency mapping.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 31 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (16)
    Reported As
    Symbol Synonym
    Met
    (Ameku et al., 2018, Shin et al., 2018, Wu et al., 2018, Baumann et al., 2017, He et al., 2017, Kang et al., 2017, Miyakawa and Iguchi, 2017, Rahman et al., 2017, Schwenke and Lazzaro, 2017, Transgenic RNAi Project members, 2017-, Bontonou et al., 2015, Jindra et al., 2015, Schertel et al., 2015, Ugrankar et al., 2015, Wen et al., 2015, He et al., 2014, Liu et al., 2014, Mirth et al., 2014, Victorsen and White, 2014.5.13, Koyama et al., 2013, Yamanaka et al., 2013, Bielska et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Riddiford, 2012, Abdou et al., 2011, Abdou et al., 2011, Dubrovsky et al., 2011, Li et al., 2011, Baumann et al., 2010, Baumann et al., 2010, Riddiford et al., 2010, Zhang et al., 2010, Liu et al., 2009, Barry et al., 2008, Flatt et al., 2008, Jindra and Konopova, 2008, Jindra and Flatt, 2007.4.13, Wang et al., 2007, Flatt et al., 2006, Godlewski et al., 2006, Wilson et al., 2006, Wilson et al., 2006, Flatt and Kawecki, 2005, Flatt et al., 2005, Miura et al., 2005, ffrench-Constant et al., 2004, Flatt and Kawecki, 2004, Adam et al., 2003, Wilson et al., 2003, Wilson et al., 2002, Wilson et al., 2001, Gilbert et al., 2000, Gruntenko et al., 2000, Gruntenko et al., 2000, Pursley et al., 2000, Gruntenko et al., 1999, Pursley et al., 1999, Wilson et al., 1999, Ashok et al., 1998, Feyereisen, 1998, Hahn, 1998, Restifo and Wilson, 1998, Wilson and Ashok, 1998, Wilson et al., 1998, Wilson et al., 1997, Wilson, 1996, Henrich and Brown, 1995, Turner and Wilson, 1995, Morton, 1993, Riddiford, 1993, Segal, 1993, Minkoff and Wilson, 1992, Riddiford and Ashburner, 1991, Shemshedini and Wilson, 1990, Shemshedini et al., 1990, Wilson and Fabian, 1986)
    Mett
    Secondary FlyBase IDs
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (139)