General Information
Symbol
Dmel\His3.3AUASp.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0280301
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
UASp regulatory sequences are fused upstream of a FRT cassette containing the His3.3A coding region tagged with GFP. The cassette is followed by the His3.3A coding region tagged with the mKO fluorescent protein.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
In the absence of Scer\FRT, the T:Avic\GFP-tagged His3.3A coding region in P{UASp(FRT.His3.3A-GFP)His3.3A-mKO} is expressed under the control of the UASp sequences and there is little expression of the T:Zzzz\KOmKO-tagged His3.3A coding region which is downstream of the Scer\FRT cassette. In the presence of Scer\FRT, the Scer\FRT cassette containing the T:Avic\GFP-tagged His3.3A coding region is excised, allowing expression of the T:Zzzz\KOmKO-tagged His3.3A coding region under the control of the UASp sequences. This dual-colour method can be used to differentially label "old" versus "new" histones in cells.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
His3.3AScer\UAS.P\T.T:Avic\GFP
His3.3AUASp.GFP
Name Synonyms
Secondary FlyBase IDs
    References (1)