General Information
Symbol
Dmel\Ack86
Species
D. melanogaster
Name
FlyBase ID
FBal0269978
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
Boundaries are approximate Ack86 is described as a 2.7 kb deletion resulting from the imprecise excision of P{SUPor-P}KG00869 that removes the first and part of the second exon of Ack and extends to 54 bases after the ATG start codon. It's not clear if the upstream boundary is at the insertion site or beyond.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
2.7kb deletion extending from the beginning of the second exon of Ack (54bp after the ATG start codon).
Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of Ack.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Most homozygous flies eclose with no morphological defects. Homozygous females are fertile. Homozygous males are completely sterile and their seminal vesicles contain no sperm. The maintenance of germ stem cells and germ cell cytokinesis appears normal in the mutant testes. Properly coiled spermatids are not detected in the mutant testes. The total number of elongated cysts in the mutant testes is normal, but the number of pre-investment cone assembly cysts is reduced, and the number of cysts in which the investment cone has departed is increased compared to wild type. This suggests that the spermatids proceed to individualisation early but fail to coil properly, resulting in an accumulation of cysts in which the investment cone has departed.
The individualisation complexes are displaced from the outer region of the testis coil to the inner region in mutant individualising spermatids. No mature sperm are present in the seminal vesicle in mutant males. Homozygotes have slightly smaller eyes than normal.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Ack86 has male sterile phenotype, non-suppressible by Mmus\Tnk1βTub85D.FL
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Ack86/Ack86 is an enhancer of eye phenotype of hidGMR.PG/WGMR.PG
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Ack-likeβTub85D.FL.T:Avic\GFP fails to rescue the sterility of Ack86 males.
Homozygosity for Ack86 enhances the reduced eye size caused by WGMR.PG. Ack86/+ flies expressing rprGMR.PH fail to eclose. The pharate adults have eyes that are partially ablated. The ablated eye area is replaced by a hole in the head cuticle where eye tissue would normally reside and the optic lobe region of the brain is visible. The few female adult escapers (no male escapers are seen) have an eye that is slightly reduced in size compared to flies expressing rprGMR.PH in a wild-type background.
dpphr27/+ ; Ack86/Ack86 embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpphr27/+ or Ack86/Ack86 single mutant embryos. dpphr92/+ ; Ack86/Ack86 embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpphr92/+ or Ack86/Ack86 single mutant embryos.
Xenogenetic Interactions
Statement
Reference
Mmus\Tnk2βTub85D.FL restores fertility and sperm formation in Ack86 males. Mmus\Tnk1βTub85D.FL fails to rescue the sterility of Ack86 males.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)