General Information
Symbol
Dmel\nst16923
Species
D. melanogaster
Name
FlyBase ID
FBal0267101
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
An 8 base pair duplication, leading to a frameshift producing a premature STOP codon after Asn218.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Embryos maternally and zygotically mutant for nst16923 exhibit defects in mesoderm layer formation during gastrulation, and generation of the dorsal tracheal trunk is completely abolished. Differentiation of eve-positive dorsal mesoderm precursors is also affected in embryos maternally and zygotically mutant for nst16923, but is unaffected in zygotic mutants. The amount of UDP-HexNAc in embryos derived from nst16923 germline clones is only 17% of that in wild type embryos. Global O-GlcNAcylation is also reduced. Injection of GlcNAcstatin C (a small molecular inhibitor of Oga that promotes O-GlcNAcylation) into embryos derived from nst16923 germline clones suppresses the mesoderm defect and significantly increases the number of eve-positive dorsal mesoderm precursors compared to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
nst16923 is an enhancer of mesoderm phenotype of htlYY262
Additional Comments
Genetic Interactions
Statement
Reference
nst16923 zygotically enhances the reduced eve-positive dorsal mesoderm precursor phenotype of htlYY262 embryos. Expression of htlScer\UAS.T:λ\cI-DD using Scer\GAL4twi.PU fails to rescue mesoderm differentiation in embryos derived from nst16923 germline clones. Expression of sxcScer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU partially suppresses the mesoderm phenotype of embryos derived from nst16923 germline clones.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of nsthsp.PM rescues the mesoderm and tracheal phenotypes, as well as the lethality, of nst16923 germline clone-derived embryos. nsthsp.PM restores UDP-HexNAc levels in nst16923 embryos to 80% that of wild type. Expression of nstScer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU or Scer\GAL4btl.PS suppresses the mesoderm and tracheal defects, respectively, of embryos derived from nst16923 germline clones. Expression of nstS86A.Scer\UAS.T:Ivir\HA1 using Scer\GAL4twi.PU or Scer\GAL4btl.PS does not suppress the mesoderm or tracheal defects, respectively, of embryos derived from nst16923 germline clones.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)