General Information
Symbol
Dmel\LrrkY1383C.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0220787
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Comment:
The Y1383C mutation was reported relative to Lrrk-PB. analogous mutation in human LRRK2 implicated in Parkinson;disease 8; mutation carried on in vitro construct; site of nucleotide substitution in fly gene and specific disease association inferred by FlyBase curator.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: Y1383C.
UAS regulatory sequences drive expression of a mutated form of Lrrk.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 1 )
Disease
Evidence
References
inferred from mutant phenotype
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Axonal transport of mitochondria is disrupted in larval motor neurons expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4D42. Flies expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4D42 show locomotor defects.
Flies expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).
60 day old adults expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4ple.PF show a significant reduction in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster compared to control flies, although the number of neurons in the protocerebral posterior medial 3 cluster is unaffected in the mutant flies. Flies expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4da.G32 show significantly higher sensitivity to paraquat and to hydrogen peroxide compared to control flies.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The severity of the eye defects caused by expression of foxoScer\UAS.cKb under the control of Scer\GAL4GMR.PF are enhanced by co-expression of LrrkY1383C.Scer\UAS. Co-expression of foxoScer\UAS.cKb and LrrkY1383C.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 throughout the lifespan results in a reduction in lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons.
The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4ple.PF is suppressed by co-expression of ThorScer\UAS.cMa.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
LrrkY1383C.Scer\UAS
LrrkY1383C.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)