General Information
Symbol
Dmel\DifdsRNA.IR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0196036
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of an inverted repeat of a 500bp cDNA fragment from the 5' end of the Dif coding sequence.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4c564 blocks the normal inflammatory response to Gram-positive bacterial infection (assessed by the expression levels of Drosomycin), but has no significant effect in basal (non-infected) conditions, as compared to controls.
Flies expressing DifdsRNA.IR.Scer\UAS, under the control of Scer\GAL4da.G32 exhibit impaired immune response . They exhibit a dramatic loss of survival rate after infection with E.faecalis (gram-positive bacterium). This is mirrored to a lesser extent, when these flies are infected with B.bassiana (filamentous fungus) or C.albicans (yeast).
Adult flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 show a reduced survival rate compared to control flies after bacterial (E.faecalis) infection.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of either MP1dsRNA.Scer\UAS.WIZ or Sp7dsRNA.Scer\UAS.WIZ results in a further decrease in the survival rate of flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 after fungal (C.albicans) infection.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DifdsRNA.IR.Scer\UAS
DifdsRNA.IR.UAS
Name Synonyms
Secondary FlyBase IDs
    References (3)