Over-expression of Vps35EY14200
driven by Scer\GAL4GMR.PU
causes a mild eye phenotype with occasional presence of black lesions (in around 8% of mutant flies, compared to 3% in controls). Expression of Vps35EY14200
driven by Scer\GAL4Ddc.PU
does not cause significant locomotor deficits or changes in lifespan.
Rare adult homozygous escapers are seen, but they are unable to move and die shortly after eclosion.
Homozygous larvae develop melanotic masses in the body cavity. The hemolymph of mutant larvae contains an increased number of plasmatocytes compared to controls and also contains significant numbers of lamellocytes (which are absent in wild-type larval haemolymph).
larvae have an increased number of haemocytes compared to controls.
Primary cultured haemocytes from homozygous and Vps35EY14200
third instar larvae show reduced uptake of maleylated bovine serum albumin (mBSA) compared to wild-type haemocytes, indicating a defect in endocytosis.
Haemocytes isolated from mutant larvae often have increased numbers of lamellopodia compared with wild type and these often appear detached from the coverslip beneath them.
Boutons at the Vps35EY14200
neuromuscular junction show no significant defect in uptake of the styryl dye FM1-43FX, during stimulation by 90mM K[+].
Neuromuscular junctions (NMJs) of homozygous larvae have many satellite boutons that protrude from larger boutons (these are rare in wild-type synapses). This results in a twofold increase in total bouton number per NMJ compared to wild type.
NMJs have an increase in total bouton number per NMJ compared to wild type.