General Information
Symbol
Dmel\Vps35EY14200
Species
D. melanogaster
Name
FlyBase ID
FBal0161256
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion 21bp upstream of the first and 190bp upstream of the second ATG site/
Insertion in the 5' untranslated region.
Insertion components
P{EPgy2}Vps35EY14200
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 1 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Over-expression of Vps35EY14200 driven by Scer\GAL4GMR.PU causes a mild eye phenotype with occasional presence of black lesions (in around 8% of mutant flies, compared to 3% in controls). Expression of Vps35EY14200 driven by Scer\GAL4Ddc.PU does not cause significant locomotor deficits or changes in lifespan.
Rare adult homozygous escapers are seen, but they are unable to move and die shortly after eclosion. Homozygous larvae develop melanotic masses in the body cavity. The hemolymph of mutant larvae contains an increased number of plasmatocytes compared to controls and also contains significant numbers of lamellocytes (which are absent in wild-type larval haemolymph). Vps35EY14200/Df(2R)ED3952 larvae have an increased number of haemocytes compared to controls. Primary cultured haemocytes from homozygous and Vps35EY14200/Df(2R)ED3952 third instar larvae show reduced uptake of maleylated bovine serum albumin (mBSA) compared to wild-type haemocytes, indicating a defect in endocytosis. Haemocytes isolated from mutant larvae often have increased numbers of lamellopodia compared with wild type and these often appear detached from the coverslip beneath them. Boutons at the Vps35EY14200 neuromuscular junction show no significant defect in uptake of the styryl dye FM1-43FX, during stimulation by 90mM K[+]. Neuromuscular junctions (NMJs) of homozygous larvae have many satellite boutons that protrude from larger boutons (these are rare in wild-type synapses). This results in a twofold increase in total bouton number per NMJ compared to wild type. Vps35EY14200/Df(2R)ED3952 NMJs have an increase in total bouton number per NMJ compared to wild type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Vps35EY14200 has neuroanatomy defective | larval stage phenotype, non-suppressible by fz2[+]/fz2C1
Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Vps35EY14200 has NMJ bouton phenotype, non-suppressible by fz2[+]/fz2C1
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
The increased bouton number at the neuromuscular junction that is seen in homozygous Vps35EY14200 larvae is not suppressed by fz2C1/+ but is suppressed by witunspecified/+ or Mad10/+. The increased bouton number at the neuromuscular junction that is seen in Vps35EY14200/Df(2R)ED3952 larvae is suppressed by Rac1J11/+. The increased bouton number at the neuromuscular junction that is seen in Vps35EY14200/Df(2R)ED3952 larvae is suppressed by expression of Rac1N17.Scer\UAS under the control of either Scer\GAL4elav.PLu or Scer\GAL4C57.
Xenogenetic Interactions
Statement
Reference
Co-expression of Vps35EY14200 significantly suppresses eye phenotypes (presence of black lesions in around 50% of flies), locomotor deficits and shortened lifespan in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4GMR.PU at 29[o]C. Co-expression of Vps35EY14200 significantly suppresses locomotor deficits in flies with expression of Hsap\LRRK2I1122V.Scer\UAS or Hsap\LRRK2Y1699C.Scer\UAS driven by Scer\GAL4Ddc.PU.
Complementation and Rescue Data
Comments
Expression of Vps35EY14200 under the control of Scer\GAL4Hml.PG rescues the endocytic defects seen in cultured Vps35EY14200/Df(2R)ED3952 haemocytes in the absence of a Scer\GAL4 driver. Expression of Vps35EY14200 under the control of Scer\GAL4Hml.PG rescues the increased number of haemocytes seen in Vps35EY14200/Df(2R)ED3952 larvae in the absence of a Scer\GAL4 driver. The increased bouton number at the neuromuscular junction that is seen in Vps35EY14200/Df(2R)ED3952 larvae in the absence of a Scer\GAL4 driver is partially rescued by expression of Vps35EY14200 under the control of one of Scer\GAL4elav.PLu or Scer\GAL4C57 and is fully rescued by expression of Vps35EY14200 using both Scer\GAL4elav.PLu and Scer\GAL4C57 drivers simultaneously.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CG5625EY14200
Vps35EY14200
Name Synonyms
Secondary FlyBase IDs
    References (5)