homozygous flies show a consistent and significant shortening of the period of the behavioural rhythm compared to wild-type controls under free-running conditions.
mutants exhibit largely normal behavioural rhythms.
mutant flies exhibit a similar avoidance response to benzaldehyde as in wild-type.
mutants display normal eclosion behaviour.
mutants exhibit axon guidance defects. The overall pattern of the CNS axon scaffold is fairly normal; the longitudinal axon tracts as well as the anterior and posterior commissures are spaced normally and usually have normal thickness. The main defect observed is related to the anterior defasciculation of the longitudinal tracts, whereby in certain segments up to five axonal bundles can be observed. in addition, commissural bundles in some segments appear slightly abnormal and wander between commissures, extending their projections along and over the midline instead of projecting towards the contralateral side, resulting in a distinct phenotype. Quantification reveals that the most prominent defect in roboBG01092
individuals is the defasciculation of longitudinal axon bundles.
responds to a light pulse during the early night (ZT 15) with an approximate 3hr delay, as in wild-type, although the phase shift in mutant flies is larger than in wild-type. After ZT 21, the magnitude of the hase respose is slightly smaller in roboBG01092
individuals compared to wild-type.
After 10 days under synchronizing temperature cycles (25-20[o]C, where the warmer temperatures are reminiscent of the light period), where roboBG01092
flies disply close to 24hr rhythms under temperature entrainment, as wild-type, the endogenous free-running period is significantly shorter in roboBG01092
homozygotes than in wild-type controls
mutants display a similar morning anticipation as wild-type in a 12hr LD regime. However the anticipation of the evening transition starts at least half an hour earlier than in wild-type controls, consistent with the shorter periodicity in the activity rhythms in free-running conditions.
Small LNv neurons are mislocalised in roughly half of the roboBG01092
brain hemispheres analysed. Misplaced neurons are located in the dorsal protocerebrum and occasionally found next to the large LNvs. Supernumerary cells are not detected in pdf
-positive clusters roboBG01092
brains. Subtly altered axon trajectories are found in a high proportion of the dissected roboBG01092
brains. The axon bundle running along the posterio optic tract does not run in the same way in roboBG01092
brains as in wild-type. To quantify this difference the angle between the initial turn of the axon fibre and a base line with the same origin that reaches to an intermediate point in the posterior optic tract just on top of the esophageal foramen at the midline. Using this assay, wild-type brains tend to display 'negative' angles, while most roboBG01092
brains exhibit 'positive' angles.
brains show no apparent difference in the trajectory of photoreceptor axons at the level of the optic lobe.
is homozygous viable.
transheterozygotes exhibit ectopic midline crossing.