General Information
Symbol
Dmel\Droncpro.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0104745
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dronc, UAS-pro-dronc, UAS-proDronc, UAS-droncWT
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
The complete coding sequence of Dronc from nucleotides 369 to 1721 is expressed under the control of UASt sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF results in pigment cell death.
Overexpression of Ncpro.Scer\UAS in the eye-antennal disc under the control of Scer\GAL4GMR.PF induces cell death. The adult eyes appear normal-sized, and show a 'spotty-pigmented-eye' phenotype. Overexpression of Ncpro.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF does not affect cell division but slightly increases apoptosis compared to wild-type.
Expression of Ncpro.Scer\UAS under the control Scer\GAL4c42 results in semi-lethality during larval and pupal development. The morphology of larval Malpighian tubules is disrupted in these animals. Tubules show reduced size, number, and some segments are missing. Other tubules appear to be reduced in length.
Scer\GAL4GMR.PF-mediated expression of a weak Ncpro.Scer\UAS results in adult eyes with normal external morphology but with internal ablation of most photoreceptor and pigment cells. Eyes show only very small patches of red pigmentation. Expression of a strong Ncpro.Scer\UAS results in more severely malformed and depigmented eyes. At 25[o]C, Scer\GAL4GMR.PF-mediated expression of the strong NcDeltaN.Scer\UAS fail to eclose out of the pupal case. At 18[o]C, a few flies emerge with completely ablated eyes and reduced head size.
Flies carrying a weakly expressed Ncpro.Scer\UAS line driven by Scer\GAL4GMR.PF show near regular arrays of ommatidia, but show uneven pigmentation of the eye. No pseudopupil image is found in these flies. Flies carrying a strongly expressed Ncpro.Scer\UAS line driven by Scer\GAL4GMR.PF have severely degenerated and depigmented eyes with reduced head size. The majority of these animals die as pharate adults.
Expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PU induces apoptosis in the third larval instar eye disc.
Expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF results in loss of pigment cells in the eye.
When driven by Scer\GAL4GMR.PF Jafrac2AKP.Ub.GMR causes a 'spotted eye' phenotype, exhibiting occasional red spots, despite being in a w- background.
When expression of Ncpro.Scer\UAS is driven by Scer\GAL4GMR.PF, A "spotted eye" phenotype is seen in the weak insertion P{UAS-pro-dronc}W. The eyes are mostly white, but with occasional red spots. External morphology of the eye is normal, however internally the pigment cells and photoreceptors are abnormal: Only remnants of pigment cells and vacuole like structures remain. There is a dramatic increase in numbers of apoptotic cells in the eye discs of pupae. The remnant pigment cells cause the red spot phenotype. In the stronger insertion P{UAS-pro-dronc}S flies exhibit roughened eyes that are reduced in size. Their surface morphology is distorted, disrupted and rough. The eyes are white, and the internal morphology of photoreceptors is completely disrupted. An increase in apoptosis is seen in larval eye discs. The addition of thGMR.PH to Ncpro.Scer\UAS,Scer\GAL4GMR.PF flies completely rescues the phenotypes seen in these flies. The addition of Df(3L)th102 to Ncpro.Scer\UAS,Scer\GAL4GMR.PF flies produces flies that display severely distorted eyes and die trapped in their pupal cases. The addition of BacA\p35GMR.PH to Ncpro.Scer\UAS,Scer\GAL4GMR.PF weakly suppresses the phenotypes seen in those flies, leading to a slightly increased eye size.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Embryos co-expressing Droncpro.Scer\UAS and DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in neurons under the control of Scer\GAL4elav.PU exhibit increased engulfment of apoptotic neurons.
The combined expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF generates a potent eye ablation phenotype. A BruceE81 or BruceE23 heterozygous background does not affect the pigment cell death phenotype caused by the overexpression of Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF. Either a BruceE81/+ or BruceE23/+ heterozygous background has no effect on the eye ablation phenotype found upon co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF.
Co-expression of Ncpro.Scer\UAS in ykiScer\UAS.cHa-expressing cells (both under the control of Scer\GAL4GMR.PF) results in a decrease in cell division and an increase in apoptosis, compared to expression of ykiScer\UAS.cHa alone. Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS together, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal discs and adult eyes. Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC, Ncpro.Scer\UAS and hpoScer\UAS.cUa in the developing eye, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal disc and adult. This phenotype appears stronger than controls as both photoreceptor and pigment cells are eventually eliminated in animals co-expressing hpoScer\UAS.cUa.
Expression of Ncpro.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation.
Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in flies expressing either the weak or strong Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of P{Sym-UAS-Hsrω} rescues the pupal lethality and small head phenotypes seen in flies expressing the strong Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF.
Co-expression of Hsp60DdsRNA.Sym.Scer\UAS suppresses the eye phenotypes caused by both weakly and strongly expressing Ncpro.Scer\UAS lines driven by Scer\GAL4GMR.PF, and all these animals eclose with normal head size.
Homozygosity for Ice17 does not suppress the apoptosis induced in the third larval instar eye disc by expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PU.
The loss of pigment cells in the eye caused by expression of Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by effD73/+.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The expression of Droncpro.Scer\UAS under the control of Scer\GAL4nos.PU rescues both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of testes observed in DroncI29/DroncL32 transheterozygotes.
Expression of NcdsRNA.Scer\UAS.cLa in animals expressing Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF rescues the eye phenotype observed when Ncpro.Scer\UAS is expressed alone.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Droncpro.Scer\UAS
Droncpro.UAS
Ncpro.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (20)