General Information
Symbol
Dmel\robo1UAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kidd
FlyBase ID
FBal0086358
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-robo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
A robo1 cDNA containing the entire open reading frame but lacking most of the untranslated regions is expressed under the control of UAS regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
antennal glomerulus DM2 & olfactory neuron, with Scer\GAL4lz-gal4
antennal glomerulus DM6 & olfactory neuron, with Scer\GAL4lz-gal4
tergal depressor of trochanter muscle motor neuron & dendrite, with Scer\GAL4shakB.lethal.4.1
Detailed Description
Statement
Reference
The expression of robo1Scer\UAS.cKa under the control of Scer\GAL4elav.PU does not cause any obvious axon guidance defects in the longitudinal connective, as compared to controls.
Expression of roboScer\UAS.cKa in MN-VO4-6 under the control of Scer\GAL4eve.CQ2 abolishes dendritic targeting to the midline (with 20% penetrance). Expression of roboScer\UAS.cKa selectively in MN-LL1 and MN-DA3 under the control of Scer\GAL4eve.CQ2 reliably converts the MNLL1 dendritic tree to a MN-DA3 morphology in 78% cases and manipulates the existing MN-DA3 to enhance the characteristic lateral confinement of its dendrites in 80% of cases. Expression of roboScer\UAS.cKa does not lead to statistically significant changes in overall dendritic length or tip number. Expression of roboScer\UAS.cKa under the control of Scer\GAL4eve.CQ2 reduces innervation of the intermediate neuropile and leads to a greater proportion of the arbor to be located in the lateral territory.
Expression of roboScer\UAS.cKa in cardioblasts using Scer\GAL4Mef2.PR does not result in any observable defects in the heart tube.
The longitudinal tracts are positioned much more laterally than normal in embryos expressing roboScer\UAS.cKa under the control of Scer\GAL4elav.PLu.
In roboScer\UAS.cKa; Scer\GAL4eg-Mz360 embryos, serotonergic axons cross this midline as normal, and these neurons retain their normal ability to take up serotonin.
The antennal commissure forms normally in roboScer\UAS.cKa(3X); Scer\GAL4SG18.1 flies, but antennal glomeruli are abnormal. In the antennal lobes of roboScer\UAS.cKa; Scer\GAL4lz-gal4 flies, DL3 and DM6 glomerulus innervating neurons are shifted to more central locations. These neurons frequently project into only one lobe.
When roboScer\UAS.cKa is driven by Scer\GAL4ap-md544 Tv axons are redirected from the dorsal neurohemal organs.
Embryos overexpressing roboScer\UAS.cKa under the control of Scer\GAL4ftz.ng show normal Fas2-axon projections.
94% of ganglionic branches expressing roboScer\UAS.cKa under the control of Scer\GAL4bs.Term turn prematurely.
Expression of roboScer\UAS.cKa, under the regulation of Scer\GAL4A307 deflects the giant fiber axon trajectory laterally with 100% penetrance. The giant fiber axons deflect mildly in the posterior half of the first thoracic neuromere and in the target area in the second thoracic neuromere. Expression of two copies of roboScer\UAS.cKa, under the regulation of Scer\GAL4A307 results in a greater lateral displacement compared to expression of one copy alone, demonstrating a dosage dependency in the robo system. Expression of roboScer\UAS.cKa under the control of Scer\GAL4A307 can result in a "bendless"-like phenotype (the end of the giant fiber (GF) is either swollen or tapered). This is also observed to a lesser extent when roboScer\UAS.cKa expression is regulated by Scer\GAL4c17. Expression of roboScer\UAS.cKa, under the control of Scer\GAL4A307 results in the disconnection of approximately one-fourth of the giant fibers from the tergotrochanteral motor neurons, while another one-fourth to one-half of the flies exhibit an increased response latency and are not able to follow stimuli given with a frequency of 100Hz. Ectopic expression of roboScer\UAS.cKa, under the control of Scer\GAL4shakB.lethal.4.1, in the tergotrochanteral motor neurons results in stunted tergal depressor of trochanter muscle motor neuron & dendrite s with a penetrance of 100%. The medial tergal depressor of trochanter muscle motor neuron & dendrite s do not reach the midline and appear stalled and distorted 20-30μm lateral to the midline. The lateral tergal depressor of trochanter muscle motor neuron & dendrite is often absent or abnormal in these animals as well. Expression of roboScer\UAS.cKa simultaneously presynaptically (under the control of Scer\GAL4A307) in the giant fibers and postsynaptically (under the control of Scer\GAL4shakB.lethal.4.1), in the tergal depressor of trochanter muscle motor neuron generates an increase in the number of completely wild-type connections and an increase in the number of completely disconnected synapses. Expression of roboScer\UAS.cKa only in the motor neuron (under the control of Scer\GAL4shakB.lethal.4.1) results in completely aberrant connections, with no wild-type synaptic connections. Coexpression both presynaptically and postsynaptically results in 22% wild-type flies.
Expression of roboScer\UAS.cKa under the control of Scer\GAL4GMR.PF has little if any effect on retinal patterning. Expression of roboScer\UAS.cKa under the control of Scer\GAL4elav-C155 results in a low level of guidance errors in the ISNb.
Expression of roboScer\UAS.cKa under the control of Scer\GAL4how-24B results in guidance defects in muscles 21 through 23, but no other significant muscle patterning defects.
50% of lateral transverse muscles 21 to 23 expressing roboScer\UAS.cKa under the control of Scer\GAL4how-24B are attracted to the segment borders at sites that express high levels of sli.
Animals that express a relatively high transgene dose of roboScer\UAS.cKa driven by Scer\GAL4elav.PLu have a mild phenotype in which some commissures appears somewhat thinner.
Axons do not cross the midline in embryos expressing roboScer\UAS.cKa under the control of Scer\GAL4elav.PLu. Three distinct Fas2-expressing longitudinal pathways on each side of the midline are still seen. Expression of roboScer\UAS.cKa under the control of Scer\GAL4ap-md544 leads to no alteration in the lateral position of the ap-expressing neurons. Expression of leaEP2582 under the control of Scer\GAL415J2 sometimes results in either the dMP2 or vMP2 axon being found in the intermediate Fas2-expressing pathway (they normally extend in the medial pathway).
No axons cross the midline in the central nervous system of embryos expressing roboScer\UAS.cKa under the control of Scer\GAL4elav.PLu.
Embryos expressing roboScer\UAS.cKa under the control of Scer\GAL4elav.PLu have subtle fasciculation defects, although the overall axon scaffold appear quite normal.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
The expression of robo1Scer\UAS.cKa under the control of Scer\GAL4elav.PU does not suppress the defects in the longitudinal connective observed in late mmyslm homozygous embryos.
The expression of robo1Scer\UAS.cKa under the control of Scer\GAL4Mef2.247 partially suppresses the decreased filopodial and lamellopodial activities, but not the slow migration, displayed by fra3 homozygous embryonic heart cardioblasts; this expression also rescues the the associated embryonic heart lumen formation defects of fra3 homozygotes. The expression of robo1Scer\UAS.cKa under the control of Scer\GAL4Mef2.247 does not suppress the embryonic heart cardioblast defects (i.e. rounded cell morphology, decreased migration velocity, and decreased filopodial and lamellopodial activities) observed in robo11, robo2lea-2 double homozygotes.
Overexpression of roboScer\UAS.cKa under the control of Scer\GAL4ftz.ng in Abl4 homozygous mutants suppresses the frequency of abnormal crossovers observed in Abl4 mutants. Overexpression of roboScer\UAS.cKa under the control of Scer\GAL4ftz.ng in Abl2 homozygous mutants suppresses the frequency of abnormal crossovers observed in Abl2 mutants.
When commScer\UAS.cKa is coexpressed with roboScer\UAS.cKa, under the control of Scer\GAL4shakB.lethal.4.1,in the tergal depressor of trochanter muscle motor neuron, one copy of commScer\UAS.cKa is able to suppress the anatomical and physiological effect of one or two copies of roboScer\UAS.cKa. The lateral shifts in the giant fibers induced by overexpression of roboScer\UAS.cKa under the control of Scer\GAL4A307 can be suppressed by co-overexpression of commScer\UAS.cKa with Scer\GAL4A307. When commScer\UAS.cKa is coexpressed with roboScer\UAS.cKa, the lateral displacement of the giant fiber axons is completely rescued. Coexpression of commΔC.Scer\UAS, under the control of Scer\GAL4A307, with roboScer\UAS.cKa has no effect on the robo-induced giant fiber axon deflection. Coexpression of commΔC.Scer\UAS with roboScer\UAS.cKa, under the control of Scer\GAL4shakB.lethal.4.1 has no effect on the ability of robo to disrupt the dendritic guidance of the tergotrochanteral motor neurons.
Co-expression of roboScer\UAS.cKa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of AblScer\UAS.cFa and roboScer\UAS.cKa under the control of Scer\GAL4elav-C155 dramatically increases the frequency of the ISNb bypass phenotype seen when each is expressed alone under the control of Scer\GAL4elav-C155.
87% of segments show crossing of the midline by muscles 6/7 in robo1 leaX123 double mutant embryos. This defect is rescued by expression of roboScer\UAS.cKa under the control of Scer\GAL4how-24B, but the axonal phenotype of these embryos is mutant.
The lateral transverse muscles make normal attachments in 98% of segments in embryos expressing roboScer\UAS.cKa under the control of Scer\GAL4how-24B that are also mutant for sli2.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The expression of robo1Scer\UAS.cKa under the control of Scer\GAL4Mef2.247 rescues the robo11 homozygous embryonic heart cardioblast defects (i.e. migration velocity and in filopodial and lamellopodial extensions and activities) and embryonic heart lumen formation defects.
The ectopic midline innervation phenotype found in robo1/robo2 mutants can be rescued by expression of roboScer\UAS.cKa under the control of Scer\GAL4eve.CQ2.
Expression of roboScer\UAS.cKa under the control of Scer\GAL4bs.Term rescues the crossing of the midline phenotype that occurs in the ganglionic branches of robounspecified embryos and instead, 91% of the ganglionic branches that express robo in GB1 turn to migrate posteriorly prematurely. Expression of roboScer\UAS.cKa under the control of Scer\GAL4elav-C155 significantly rescues the axon midline crossing phenotype seen in robounspecified embryos, but 21% of the ganglionic branches still cross the midline in these embryos.
Expression of roboScer\UAS.cKa driven by Scer\GAL4s.gcm rescues the lateral positions of the glia but does not rescue the axonal misrouting across the midline of robo3 mutants.
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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
robo1Scer\UAS.cKa
robo1UAS.cKa
roboScer\UAS.cKa
roboUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kidd
Secondary FlyBase IDs
    References (22)